|
|
|
| Registros recuperados: 17 | |
|
|
| Lorenson,M.S.; Miani,M.; Guizzo,J.A.; Barasoul,B.; Martínez-Martínez,S.; Rodríguez-Ferri,E.F.; Gutiérrez-Martín,C.B.; Kreutz,L.C.; Frandoloso,R.. |
| ABSTRACT Glässer's disease is an emergent bacterial disease that affects swine husbandries worldwide causing important economic losses. The aetiological agent, Haemophilus parasuis, is currently divided in fifteen serovars but an increasing number of non-typeable serovars have been reported. Indirect hemagglutination (IHA) is indicated as a serotyping method for H. parasuis. In the present study, we describe an additional step that aims to work around a possible obstacle in the original protocol that may compromise the outcome of this assay. We observed that the choice of anticoagulant for blood collection influences and/or impairs spontaneous adsorption of H. parasuis antigens on sheep red blood cells (SRBCs). However, regardless of the anticoagulant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Haemophilus parasuis; Diagnosis; Tannic acid; Serotyping; Indirect hemagglutination. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000100015 |
| |
|
|
| Magalhães,Ana Paula Gomes de Oliveira; Pinto,Artur da Silveira. |
| Thirty one Streptococcus pneumoniae invasive strains were isolated from a pediatric population in Belo Horizonte from June, 1999 to May, 2001. Penicillin, trimethoprim-sulfamethoxazole, tetracycline and chloramphenicol resistance rates for the isolates were 41.9, 58.1, 25.8 and 3.2%, respectively. Intermediate penicillin resistant (MICs between 0.1 and 1.0 µg/ml) and resistant (MICs > 2.0 µg/ml) isolates occured at rates of 38.7 and 3.2%, respectively. Resistance to erythromycin, ofloxacin, rifampin or vancomicyn was not detected. Ten S. pneumoniae serotypes (14, 5, 10 A, 6B, 15B, 18C, 6 A, 18 A, 19 A and 19 F) were identified. Serotype 14 (12 out of 31) was predominant among the isolates. Penicillin and trimethoprim-sulfamethoxazole resistance was more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus pneumoniae; Antimicrobial resistance; Serotyping. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300005 |
| |
|
|
| Fica,Alberto; Bunster,Nicolás; Aliaga,Felipe; Olivares,Felipe; Porte,Lorena; Braun,Stephanie; Dabanch,Jeannette; Hormázabal,Juan Carlos; Hernández,Antonio; Benavides,María Guacolda. |
| AIMS:Bacteremic pneumococcal pneumonia (BPP) is a severe condition. To evaluate seasonal distribution, mortality, serotype frequencies, antimicrobial susceptibility, and different severity scores among patients with BPP.PATIENTS AND METHODS:Patients were identified by laboratory data and restricted to adulthood. Standard methods were used for serotyping and antimicrobial susceptibility. Risk factors were analyzed by univariate and multivariate methods. Severity scores (APACHE II, CURB-65 and CAP PIRO) were compared using ROC curves.RESULTS:Sixty events of community-acquired BPP occurred between 2005 and 2010. A seasonal pattern was detected. Mean age was 72.1 years old (81.4% >60 years). All had a predisposing factor. Previous influenza (3.3%) or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus pneumoniae; Bacteremia; Serotyping; Microbial drug resistance; Risk factors; Mortality; Elderly; CURB-65; APACHE II; PIRO. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000200115 |
| |
|
| |
|
|
| Brito,Wilia Marta Elsner Diederichsen de; Munford,Veridiana; Villaça,André Martins; Caruzo,Thabata Alessandra Ramos; Rácz,Maria-Lucia. |
| Ten faecal samples of bovine rotavirus from calves less than 30 days old from an outbreak of diarrhea in Hidrolândia, Goiás, Brazil were submitted to serological and molecular characterization, using enzyme immunoassay for subgrouping and serotyping, PAGE for determination of electropherotypes and PCR for genome typing. Nine samples belonged to group A/subgroup I rotavirus and one sample was group A / subgroup non-I/non-II. Four samples were characterized as G10P[11] (B223-like), four samples showed a mixture of two rotavirus strains (G6G10 and P[5]P[11]), one sample was characterized as G6P[11] and one sample was characterized only by G serotyping/genotyping, and did not react with any P primer used. Two electropherotypes were detected and both were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rotavirus; Diarrhea; Bovine; Genotyping; Serotyping. |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200015 |
| |
|
|
| Pelayo,Jacinta Sanchez; Elias Junior,Antonio Roberto; Lima,Nicole Ribeiro de; Navarro,Armando; Rocha,Sérgio Paulo Dejato da. |
| Abstract Ground bovine meat is commonly consumed by the population of Brazil. However, it constitutes an excellent medium for the multiplication of microorganisms due to available nutrients and handling practices prior to consumption. Here, we examined 100 samples of ground beef for the presence of diarrheagenic Escherichia coli (DEC) pathotypes by PCR, and characterized isolates by analyzing their adherence to HEp-2 cells, serotype, antimicrobial susceptibility, and phylogeny. Enteroaggregative E. coli was detected in five (5%) meat samples, Shiga toxin-producing E. coli in three (3%), and atypical enteropathogenic E. coli in two (2%). According to the phylogeny, six isolates (60%) were classified in group A, two (20%) in group B1, and two (20%) in group... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrheagenic Escherichia coli; Ground beef; Gastroenteritis; Serotyping. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100502 |
| |
|
|
| Pinto,Tatiana Castro Abreu; Costa,Natália Silva; Souza,Aline Rosa Vianna; Silva,Ligia Guedes da; Corrêa,Ana Beatriz de Almeida; Fernandes,Flavio Gimenis; Oliveira,Ivi Cristina Menezes; Mattos,Marcos Corrêa de; Rosado,Alexandre Soares; Benchetrit,Leslie Claude. |
| Streptococcus agalactiae is a common agent of clinical and subclinical bovine mastitis and an important cause of human infections, mainly among pregnant women, neonates and nonpregnant adults with underlying diseases. The present study describes the genetic and phenotypic diversity among 392 S. agalactiae human and bovine strains isolated between 1980 and 2006 in Brazil. The most prevalent serotypes were Ia, II, III and V and all the strains were susceptible to penicillin, vancomycin and levofloxacin. Resistance to clindamycin, chloramphenicol, erythromycin, rifampicin and tetracycline was observed. Among the erythromycin resistant strains, mefA/E, ermA and, mainly, ermB gene were detected, and a shift of prevalence from the macrolide resistance phenotype... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus agalactiae; Pulsed field gel electrophoresis; Epidemiology; Serotyping; Resistance profile. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000200003 |
| |
|
|
| Mendonça,Karla Sequeira; Michael,Geovana Brenner; Nalério,Élen Silveira; Mendonça,Marcelo; Cardoso,Marisa Ribeiro Itapema; Silva,Wladimir Padilha da. |
| ABSTRACT: Listeria monocytogenes is of notable concern to the food industry, due to its ubiquitous nature and ability to grow in adverse conditions. This study aimed to determine the genotypic profile of L. monocytogenes strains isolated from refrigerated chickens marketed in the southern part of Rio Grande do Sul, Brazil. The strains of L. monocytogenes isolated were characterized by serotyping and Pulsed Field Gel Electrophoresis (PFGE). Three different serotypes (1/2a, 1/2b and 4e) were evaluated by PFGE, and the macrorestriction patterns utilizing enzymes AscI and ApaI, revealed five different pulsotypes. The presence of such varied genotypic profiles demonstrates the prevalence of L. monocytogenes contamination of chicken processing environments,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contamination; Pathogen; Prevalence; Serotyping. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016000100132 |
| |
|
| |
|
|
| Darini,A.L.C.; Magalhães,V.D.; Levy,C.L.; Barth,A.L.; Coscina,A.L.. |
| In order to evaluate the resolving power of several typing methods to identify relatedness among Brazilian strains of Enterobacter cloacae, we selected twenty isolates from different patients on three wards of a University Hospital (Orthopedics, Nephrology, and Hematology). Traditional phenotyping methods applied to isolates included biotyping, antibiotic sensitivity, phage-typing, and O-serotyping. Plasmid profile analysis, ribotyping, and macrorestriction analysis by pulsed-field gel electrophoresis (PFGE) were used as genotyping methods. Sero- and phage-typing were not useful since the majority of isolates could not be subtyped by these methods. Biotyping, antibiogram and plasmid profile permitted us to classify the samples into different groups... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Enterobacter cloacae; Phage-typing; Serotyping; Ribotyping; Pulsed-field gel electrophoresis; Nosocomial infection. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000900004 |
| |
|
|
| Braga,Valeria; Vázquez,Sylvia; Vico,Victoria; Pastorino,Valeria; Mota,María Inés; Legnani,Marcela; Schelotto,Felipe; Lancibidad,Gustavo; Varela,Gustavo. |
| ABSTRACT The aim of this work was to study the prevalence of Listeria monocytogenes in foods obtained in retail shops and food industries located in Montevideo-Uruguay, and to identify the serogroups of the obtained isolates. Three-thousand one-hundred and seventy-five food samples (frozen, deli meats, ready-to-eat and cheese) were analyzed. The obtained isolates were serogrouped by multiplex PCR and serotyped by conventional procedure. Genetic comparisons were performed using pulsed-field gel electrophoresis on a sub-set of isolates belonging to the same serotype successively recovered from the same establishment. L. monocytogenes was isolated from 11.2% of samples. The highest prevalence was observed in frozen foods (38%), followed by cheese (10%). 1/2b... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Food-borne diseases; Listeriosis; Serotyping; Pulsed-field gel electrophoresis. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400689 |
| |
|
|
| Alvares,Jackelline Rodrigues; Mantese,Orlando Cesar; Paula,Alan de; Wolkers,Paula Carolina Bejo; Almeida,Viviene Vieira Prado; Almeida,Samanta Cristine Grassi; Guerra,Maria Luiza Leopoldo Silva; Brandileone,Maria Cristina de Cunto. |
| OBJECTIVES: To determine the prevalence of pneumococcal serotypes and antimicrobial susceptibility in patients with meningitis, and to evaluate the implications for vaccine coverage. METHODS: Pneumococcal strains obtained from normally sterile fluids from patients admitted with meningitis were isolated at the Hospital de Clínicas of the Universidade Federal de Uberlândia, Minas Gerais State, and sent to the Instituto Adolfo Lutz, city of São Paulo, São Paulo State, for further identification, serotyping, and antimicrobial susceptibility determination. RESULTS: From April 1999 to April 2009, 338 pneumococcal strains were isolated, and 72 obtained from patients with meningitis, were analyzed. Patients' ages varied from one month to 82.2 years (mean of 18.4 ±... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptococcus pneumoniae; Meningitis; Serotyping; Pneumococcal vaccines; Drug resistance. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000100005 |
| |
|
|
| El-Tayeb,Mohamed A.; Ibrahim,Abdelnasser S.S.; Al-Salamah,Ali A.; Almaary,Khalid S.; Elbadawi,Yahya B.. |
| Abstract Salmonella is recognized as a common foodborne pathogen, causing major health problems in Saudi Arabia. Herein, we report epidemiology, antimicrobial susceptibility and the genetic basis of resistance among S. enterica strains isolated in Saudi Arabia. Isolation of Salmonella spp. from clinical and environmental samples resulted in isolation of 33 strains identified as S. enterica based on their biochemical characteristics and 16S-rDNA sequences. S. enterica serovar Enteritidis showed highest prevalence (39.4%), followed by S. Paratyphi (21.2%), S. Typhimurium (15.2%), S. Typhi and S. Arizona (12.1%), respectively. Most isolates were resistant to 1st and 2nd generation cephalosporin; and aminoglycosides. Moreover, several S. enterica isolates... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Serotyping; Antibiotic resistance; Antimicrobials resistance determinants. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300499 |
| |
|
| |
|
|
| Rodriguez,JM; Rondón,IS; Verjan,N. |
| ABSTRACT Salmonella enterica is a large group of Gram-negative bacteria responsible for a number of foodborne infections associated with the consumption of contaminated poultry products. The hygienic status of raw chicken meat marketed at Ibague, Tolima, Colombia, is currently unknown. To address this issue, a cross-sectional study was conducted to estimate the prevalence of Salmonella spp., in raw chicken marketed at different outlets in this city. Salmonella spp. was isolated by standard microbiological methods, followed by biochemical, serological, and molecular confirmation. Additionally, risk factors associated with the presence of the bacteria were identified. The prevalence of Salmonella in raw chicken was 17.41% (47/270), and 14 different serotypes... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Isolation; Poultry; Prevalence; Risk factors; Serotyping. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000400545 |
| |
|
|
| Komninakis,S.; Fukumori,L.; Alcalde,R.; Cortina,M.; Abdala,L.; Brito,A.; Sanabani,S.; Duarte,A.J.S.; Casseb,J.. |
| The purpose of the present study was to compare the sensitivity and specificity of V3 enzyme immunoassay (solid phase EIA and EIA inhibition) and restriction fragment length polymorphism (RFLP) with the DNA sequencing "gold standard" to identify the Brazilian HIV-1 variants of subtype B and B"-GWGR. Peripheral blood mononuclear cells were collected from 61 HIV-1-infected individuals attending a clinic in São Paulo. Proviral DNA was amplified and sequentially cleaved with the Fok I restriction enzyme. Plasma samples were submitted to a V3-loop biotinylated synthetic peptide EIA. Direct partial DNA sequencing of the env gene was performed on all samples. Based on EIA results, the sensitivity for detecting B-GPGR was 70%, compared to 64% for the Brazilian... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1 subtyping; Brazilian subtype B variant; Serotyping; DNA sequencing. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000300003 |
| |
|
|
| Casseb,J.; Hong,M.A.; Gonsalez,C.; Brígido,L.F.; Duarte,A.J.S.; Michael-Hendry,R.. |
| HIV-1 variability may have an important impact on transmission and pathogenicity. Better characterization of the HIV epidemic in Brazil is necessary for the development of vaccine trials in this country. We analyzed sera from 108 HIV-1-infected volunteers from São Paulo City to determine serotype and reactivity for V3 motifs of HIV in this population, and the relationship to transmission mode. We concluded that the HIV-1 B serotype is frequent among heterosexually infected women, even in the absence of anal sex, and that two major V3 motifs, GPGR and GWGR, had similar prevalence among women (48% and 52%, respectively) and men (56% and 44%, respectively). We also observed an equal distribution of these strains regardless of their CD4+ T cell counts,... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: HIV; Serotyping; Gp 120 V3 loop; Synthetic peptides; Sexual transmission. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998001000002 |
| |
| Registros recuperados: 17 | |
|
|
|
